Jiri usoro na akpachapụ anya nke tube na-akụ oyi

Jiri usoro na kpachara anya nke Tube na-ekpo ọkụ

N'ime nnwale microbiological, a na-ejikarị otu ngwa nnwale eme ihe, ya bụ, tube nchekwa cryopreservation.Otú ọ dị, n'ihi mgbagwoju anya ha dị iche iche, mmetụta ya dịgasị iche iche.N'ihi nke a, ugbu a, ọtụtụ ụlọ nyocha na China na-eme ka tubes na-echekwa nje bacteria n'onwe ha, nke ọ bụghị nanị na-eme ka ọrụ ahụ dịkwuo elu, kamakwa n'ihi njedebe nke ọnọdụ dị iche iche, mmetụta nke ichekwa nje bacteria anaghị enwe afọ ojuju mgbe niile.

Ya mere, ọ dị mkpa ịmara usoro eji eme ihe na ụfọdụ nlezianya nke tube nke cryopreservation, ka ọ wee rụọ ọrụ dị ukwuu.

1. Usoro nke ngwa

1).Mgbe ị na-eji tube nke cryopreservation na-echekwa ihe nlele, a na-achọsi ya ike itinye tube nke cryopreservation n'ime oghere ikuku nke nitrogen mmiri mmiri ma ọ bụ friji maka nchekwa.Ọ bụrụ na a na-echekwa tube nke cryopreservation na mmiri mmiri nitrogen, e nwere ụfọdụ ihe gbasara nke puru na nitrogen mmiri mmiri ga-abanye n'ime tube nke cryopreservation.N'oge mgbake, gasification nke mmiri mmiri nitrogen ga-eduga na ahaghị nhata nke ime na mpụga nrụgide, nke nwere ike ime ka cryopreservation tube na-agbawa, na nwere ihe ize ndụ ndu.

2).Na-arụ ọrụ tube nke cryopreservation iji mee ka obi dịghachi ndụ, ma jiri ngwa nchekwa nchekwa mee ihe n'oge usoro ahụ.A na-atụ aro iyi uwe ụlọ nyocha, uwe mkpuchi owu ma rụọ ọrụ na bench ụlọ nyocha dị mma.Ọ bụrụ na ọ ga-ekwe omume, biko yiri enyo ma ọ bụ ọta ihu.Dị ka okpomọkụ ime ụlọ n'oge okpomọkụ ga-adị elu karịa nke ahụ n'oge oyi, biko kpachara anya.

3).N'oge nchekwa nke cell cryopreserved, oyi okpomọkụ nke cryopreserved tubes ga-abụ otu.Igwe oyi na-enweghị isi ga-eduga na ice jam, nke ga-egbochi nnyefe nke mmiri ọkụ n'akụkụ abụọ, si otú a na-emepụta nrụgide dị ize ndụ ma na-emebi tube na-ekpo ọkụ.

4).Ọnụ ọgụgụ nke ihe atụ oyi kpọnwụrụ agaghị agafe oke ọrụ nke tube oyi kpọnwụrụ chọrọ.

2. Ihe ndị chọrọ nlebara anya

1).Ebe nchekwa tube na-ekpo ọkụ