Nzọụkwụ mbụ maka nnwale ELISA na-aga nke ọma—ịhọrọ efere ELISA ziri ezi

NkeELISAefere bụ ngwá ọrụ dị mkpa maka ELISA, nyocha immunosorbent jikọtara enzyme.Enwere ọtụtụ ihe na-emetụta ọganihu nke nnwale ELISA.Ịhọrọ ngwá ọrụ kwesịrị ekwesị bụ nzọụkwụ mbụ.Ịhọrọ microplate kwesịrị ekwesị ga-enyere nnwale ahụ aka ịga nke ọma.

Ihe onwunwe nkeELISAefere bụ n'ozuzu polystyrene (PS), na polystyrene enwechaghị kemịkalụ nkwụsi ike na ike na-agbaze site dị iche iche nke organic solvents (dị ka aromatic hydrocarbons, halogenated hydrocarbons, wdg), na ike mebie site ike acids na alkalis.Ọ naghị eguzogide griiz ma na-agbanwe ngwa ngwa mgbe ekpughere ya na ọkụ UV.

Kedu ụdị nkeELISAefere dị?

✦ Họrọ site na agba

efere transperent:dabara adaba maka ọnụọgụ na qualitative siri ike-phase immunoassays na njide nyocha;

efere ọcha:adabara onwe-luminescence na chemiluminescence;

efere ojii:dabara maka fluorescent immunoassays na njide nyocha.

✦ Họrọ site na ijikọ ike

Efere dị ala:Na-ejikọta ya na protein site na njikọ hydrophobic elu.Ọ dabara nke ọma dị ka onye na-ebu ihe siri ike maka protein macromolecular nwere ịdị arọ molekul> 20kD.Ya protein-njikọ ike bụ 200 ~ 300ng IgG / cm2.

Efere dị elu:Mgbe ọgwụgwọ elu gasịrị, a na-emewanye ikike ijikọ protein ya nke ukwuu, na-eru 300 ~ 400ng IgG / cm2, na ịdị arọ molekul nke protein bụ isi bụ> 10kD.

✦Hazie site na udi ala

Ala dị larịị:index refractive dị ala, dabara maka nchọpụta ya na ndị na-agụ microplate;

N'okpuru:Ntugharị refractive dị elu, nke dị mma maka ịgbakwunye, na-achọsi ike, ịgwakọta na ọrụ ndị ọzọ.Ị nwere ike ịhụ mgbanwe agba ozugbo site na nlele anya na-etinyeghị ya na onye na-agụ microplate iji chọpụta ma enwere mmeghachi omume mgbochi kwekọrọ.