Usoro akọwapụtara nke omenala cell

1. Ngwá ọrụ nkịtị

1. Akụrụngwa na ime ụlọ nkwadebe

Otu igwe distilled mmiri, igwe distilled mmiri okpukpu abụọ, tank acid, oven, osi nri nrụgide, kabinet nchekwa (na-echekwa ihe anaghị agba nchara), kabinet nchekwa (na-echekwa akụkọ ịgba ọgwụ mgbochi), tebụl nkwakọ ngwaahịa.Akụrụngwa dị n'ime ime ụlọ nkwadebe ihe ngwọta: nguzozi torsion na nguzozi eletrọnịkị (na-atụ aro ọgwụ), PH mita (n'ịtụ PH uru nke omenala ngwọta), magnetik stirrer (configuring ngwọta ụlọ ịkpali ngwọta).

2. Akụrụngwa nke ime ụlọ omenala

Mmiri mmiri nitrogen tank, nchekwa kabinet (na-echekwa sundries), fluorescent oriọna na ultraviolet oriọna, ikuku purifier usoro, obere okpomọkụ refrjiraeto (- 80 ℃), ntụ oyi, carbon dioxide cylinder, akụkụ table (ndekọ ule na-ede).

3. Akụrụngwa nke a ga-etinye n'ime ime ụlọ adịghị mma

Centrifuge (sel na-anakọta), ultra-dị ọcha worktable, inverted microscope, CO2 incubator (incubating omenala), mmiri ịsa ahụ, atọ oxygen disinfection na sterilization igwe, 4 ℃ friji (itinye serum na omenala ngwọta).

2. Aseptic ọrụ

(1) Mgbochi ime ụlọ adịghị mma

1. Na-ehicha ọnụ ụlọ ahụ na-adịghị mma mgbe niile: otu ugboro n'izu, buru ụzọ jiri mmiri mgbata na-ehicha ala, hichaa tebụl, hichaa tebụl ọrụ, wee jiri 3 ‰ lysol ma ọ bụ bromogeramine ma ọ bụ 0.5% peracetic acid hichaa.

2. Mgbochi nke CO2 incubator (incubator): buru ụzọ kpochapụ ya na 3 ‰ bromogeramine, wee hichaa ya na mmanya 75% ma ọ bụ 0.5% peracetic acid, wee jiri oriọna ultraviolet na-egbuke egbuke.

3. Mgbochi tupu nnwale: gbanye oriọna ultraviolet, ikuku oxygen sterilizer atọ na usoro nchacha ikuku maka nkeji 20-30 n'otu n'otu.

4. Mgbochi mgbe nnwale ahụ gasịrị: hichaa tebụl ultra-dị ọcha, tebụl dị n'akụkụ na ogbo microscope tụgharịrị na mmanya 75% (3 ‰ bromogeramine).

Nkwadebe sterilization nke ndị ọrụ ụlọ nyocha

1. Jiri ncha saa aka.

2. Yiri uwe kewapụrụ iche, mkpuchi iche, ihe mkpuchi na slippers.

3. Jiri bọọlụ mmanya mmanya 75% hichaa aka.

Ngosipụta nke ọrụ adịghị mma

1. All karama mmanya, PBS, omenala usoro na trypsin webatara n'ime ultra-dị ọcha workbench kwesịrị ihichapụ na 75% mmanya na mpụta elu nke karama.

2. Na-arụ ọrụ n'akụkụ ọkụ nke oriọna mmanya.

3. A ghaghị igba ogwu tupu eji ya eme ihe.

4. A na-edobe arịa (dị ka mkpuchi karama na droppers) nke na-aga n'ihu n'ebe dị elu, ma ka na-ekpo ọkụ mgbe a na-eji ya eme ihe.

5. Ọrụ niile kwesịrị ịdị nso na oriọna mmanya na-aba n'anya, ihe omume ahụ kwesịrị ịdị ọkụ na nke ziri ezi, na e kwesịghị imetụ ya aka.Ọ bụrụ na ahihia enweghị ike imetụ tankị mmiri mkpofu ahụ aka.

6. Mgbe ị na-achọ ihe karịrị ụdị abụọ nke mmiri mmiri, ṅaa ntị n'iji dochie ọkpọ ọkpọ iji gbochie mmetọ obe.

Lee isiakwụkwọ na-esote maka mkpochapụ nke ngwa.