Nchịkọta ihe atụ, nchekwa na njem chọrọ maka nnwale nkịtị

Nchịkọta ihe atụ, nchekwa na njem chọrọ maka nnwale nkịtị

1. Mkpokọta na ichekwa ụdị ọrịa pathological:

☛ Akụkụ oyi kpọnwụrụ: Wepu ihe mgbochi anụ ahụ kwesịrị ekwesị ma chekwaa ya na nitrogen mmiri mmiri;

☛ Nkebi paraffin: Wepu ihe mgbochi anụ ahụ kwesịrị ekwesị ma chekwaa ya na 4% paraformaldehyde;

☛Slides cell: A na-edozi ihe mmịfe cell na 4% paraformaldehyde maka nkeji iri atọ, wee dochie ya na PBS ma mikpuo na PBS wee chekwaa ya na 4 Celsius.

2. Mkpokọta na ichekwa ụdị ihe nleba anya mkpụrụ ndụ:

☛ anụ ahụ dị ọhụrụ: Bee ihe atụ ahụ ma chekwaa ya na nitrogen mmiri mmiri ma ọ bụ -80ºC friji;

☛Specimens paraffin: Chekwaa na ụlọ okpomọkụ;

☛Nlere ọbara dum: Were ọbara zuru oke kwesịrị ekwesị ma tinye EDTA ma ọ bụ heparin anticoagulation tube mkpokọta ọbara;

☛Ihe nlele mmiri nke ahụ: centrifugation dị elu iji nakọta sedimenti;

☛Specimens cell: A na-asachapụ mkpụrụ ndụ na TRIzol ma chekwaa ya na nitrogen mmiri mmiri ma ọ bụ -80°C friji.

3. Mkpokọta na nchekwa nke ụdị nnwale protein:

☛ anụ ahụ dị ọhụrụ: Bee ihe atụ ahụ ma chekwaa ya na nitrogen mmiri mmiri ma ọ bụ -80ºC friji;

☛Nlere ọbara dum: Were ọbara zuru oke kwesịrị ekwesị ma tinye EDTA ma ọ bụ heparin anticoagulation tube mkpokọta ọbara;

☛Specimens cell: A na-asachapụ mkpụrụ ndụ n'ụzọ zuru oke na ngwọta lysis cell wee debe ya na nitrogen mmiri mmiri ma ọ bụ -80°C friji.

4. Mkpokọta na nchekwa nke ELISA, radioimmunoassay, na ụdị nnwale biochemical:

☛Serum (plasma) sample: Were ọbara zuru oke tinye ya na tube procoagulation (tupu anticoagulation), centrifuge na 2500 rpm maka ihe dị ka nkeji 20, kpokọta ihe dị elu, ma chekwaa ya na nitrogen mmiri mmiri ma ọ bụ na friji -80 ° C;

☛Urine sample: centrifuge sample na 2500 rpm maka ihe dị ka nkeji iri abụọ, na-echekwa ya na mmiri mmiri nitrogen ma ọ bụ -80 Celsius C refrjiraeto;rụtụ aka na usoro a maka mmiri thoracic na ascites, mmiri mmiri cerebrospinal, na mmiri mmiri alveolar;

☛cell samples: Mgbe ị na-achọpụta ihe nzuzo zoro ezo, centrifuge samples na 2500 rpm maka ihe dị ka nkeji 20 ma chekwaa ha na mmiri mmiri nitrogen ma ọ bụ -80 Celsius C refrjiraeto;Mgbe ị na-achọpụta akụrụngwa intracellular, jiri PBS jiri nkwusioru cell mee ka nkwusioru cell ahụ wee kpoo ma mebie ugboro ugboro iji bibie sel ma hapụ ihe ndị dị n'ime.Centrifuge na 2500 rpm maka ihe dị ka nkeji iri abụọ ma na-anakọta supernatant dị ka n'elu;

☛Nlere anụ ahụ: Mgbe ị bechara ihe nlele ahụ, tụọ ha ma tụba ha na nitrogen mmiri mmiri ma ọ bụ friji -80ºC maka ojiji emechaa.

5. Nchịkọta ihe atụ nke metabolomics:

☛Urine sample: Centrifuge sample na 2500 rpm maka ihe dị ka nkeji 20 na-echekwa ya na mmiri mmiri nitrogen ma ọ bụ -80°C refrjiraeto;rụtụ aka na usoro a maka mmiri thoracic na ascites, mmiri ụbụrụ cerebrospinal, mmiri mmiri alveolar lavage, wdg;

☛Mgbe ị bechapụ ihe nlele anụ ahụ, tụọ ya ma tụba ya na nitrogen mmiri mmiri ma ọ bụ friji -80ºC maka ojiji emechaa;