Otu esi eji tube nke cryopreservation na sayensị na n'ụzọ ziri ezi
Ojiji nke cryopreservation tube bụ sayensị, ọ bụghị ihe dị mfe trilogy dị ka imeghe mmiri mmiri nitrogen tank, na-etinye ya na cryopreservation tube na-emechi mmiri mmiri nitrogen tank.Iji sayensị na n'ụzọ ziri ezi nke tubes cryopreservation nwere ike zere mfu nke ihe nlele ma chebe nchekwa nke ndị nyocha.
Ojiji nke cryopreservation tube bụ sayensị, ọ bụghị ihe dị mfe trilogy dị ka imeghe mmiri mmiri nitrogen tank, na-etinye ya na cryopreservation tube na-emechi mmiri mmiri nitrogen tank.Iji sayensị na n'ụzọ ziri ezi nke tubes cryopreservation nwere ike zere mfu nke ihe nlele ma chebe nchekwa nke ndị nyocha.
Igwe kefriza: nzọụkwụ na-ekpo ọkụ
Sachaa mkpụrụ ndụ ahụ na ngwọta PBS dị ọkụ, ṅụọ ngwọta ahụ, ma kpuchie sel ahụ na ngwọta nwere trypsin na EDTA (mmiri mmiri oyi akwa zuru ezu, na ntinye nke trypsin na EDTA kwesịrị ikpebi dịka eriri cell).
Tinye mkpụrụ ndụ na 37 ℃ maka nkeji 3-5.
Mgbe sel ndị ahụ kewapụrụ na ala, a na-akwụsị mmechi ahụ, a na-agbakwunye ọkara nke nwere serum, ma jiri nwayọọ kwụsịtụ ya na pipette.
Centrifuge nkwusioru cell (500 xg, 5 min) na nkwụghachi ya na ọkara nwere serum.
Ọnụ ọgụgụ cell.
Centrifuge nkwusioru cell (500 xg, nkeji 5), wepụ ihe dị elu, ma maliteghachi mkpụrụ ndụ ndị nwere ọkara nwere serum nke olu kwesịrị ekwesị.
Gwakọta mkpụrụ ndụ na ngwọta cryopreservation (60% ọkara, 20% nwa ebu n'afọ serum, 20% DMSO) na nha 1: 1, wee bufee ha na Cryo STM cryopreservation tube.Njupụta nke mkpụrụ ndụ oyi kpọnwụrụ bụ 1-5 × 106 iberibe / ml.
Cryo nwere cell STM cryopreservation tube na-atụ aro ka ajụkwa na a ọnụego nke - 1 K/min, na cryopreservation tube nwere ike tinye n'ime akpa nwere isopropanol na - 70 ℃.Ọ bụrụ na Cryo STM cryopreservation tube na-echekwa ihe atụ ndị ọzọ, nke nwere ike idowe ozugbo na -20 ℃, - 70 ℃ ma ọ bụ gas na-adọ nke mmiri mmiri nitrogen.Iji hụ na ihe nlele ahụ na-ajụ oyi n'otu n'otu, 4 ml na 5 mL Cryo The sTM cryopreservation tube kwesịrị ka etinye ya na refrjiraeto na -20 ℃ maka abalị, wee bufee ya na oge gas nke - 70 ℃ ma ọ bụ nitrogen mmiri mmiri.
Wee bufee Cryo.sTM cryopreservation tube na mmiri mmiri nitrogen tank.Iji zere mmetọ (dị ka mycoplasma) na nchekwa echiche, biko tinye Cryo.sTM cryopreservation tube na gas na-adọ nke mmiri mmiri nitrogen, ọ bụghị na mmiri mmiri na-adọ.
Kedu ka esi eji tube cryopreservation na sayensị na n'ụzọ ziri ezi?Companylọ ọrụ anyị mejupụtara ndị ọkachamara nwere nzụlite ụlọ ọrụ na ahụmịhe ahịa bara ụba iji nye ngwaahịa maka ubi nyocha sayensị ndụ na ọrụ maka ndị nyocha sayensị.Ọ nwere ike ọ bụghị nanị izute ihe pụrụ iche chọrọ nke R & D ahịa na ngwaahịa ụdị na nkwakọ ngwaahịa, ma na-egbo keukwu mkpa nke mmepụta ụlọ ọrụ na niile nkebi si obere ọnụ ọgụgụ, ọkara ọnụ ọgụgụ na nnukwu-ọnụ ọgụgụ mmepụta.
Oge nzipu: Nov-25-2022