Enwere ụdị abụọ nke ọkpọkọ friza-thaw: ụdị ntụgharị nke ime na ụdị ntụgharị mpụga.Ị̀ ma ihe dị iche n’etiti ha?
Kedu nke ka mma?Ka anyị lee anya.
A na-eji okpu mkpuchi nke dị n'ime nke cryopreservation tube mee ka ihe atụ nke ndu na-ekpo ọkụ na mmiri mmiri nitrogen.Ihe mkpuchi silicone na nozzle nke tube na-eme ka akara nke tube nke cryopreservation, nke nwere ike igbochi ntinye nke nitrogen mmiri mmiri nke ọma;
A na-eji tube mkpuchi mkpuchi mkpuchi mpụga na-ekpochapụ ihe nlele na friji.Okpu ịghasa nke okpu ntụgharị nke mpụga nwere ike ibelata ohere mmetọ mgbe ị na-eji ihe nlele
Ntugharị mpụga nwere ike imetọ karịa ntụgharị nke ime, ọ dịghịkwa mfe ịrụ ọrụ, mana ọ bụghịkwa nke ziri ezi.Lelee ọrụ ahụ.Mmiri mmiri ntụgharị dị n'ime dị mfe ịbanye na eri mkpuchi.N'ihe gbasara mgbawa tube, tube ntụgharị dị n'ime ya dị mfe ịgbawa (mana enwere ike dị ntakịrị), yabụ biko jiri nlezianya rụọ ọrụ.
Ugbu a, ka anyị mara àgwà nke n'ime ịghasa okpu nke cryopreservation tube
1. A na-emepụta okpu mkpuchi nke dị n'ime nke tube na-ekpo ọkụ maka ihe atụ na-ekpo ọkụ, na okpu eri nke mpụta ịghasa mkpuchi nwere ike ibelata ihe gbasara mmetọ mgbe ị na-eji ihe atụ.
2. A na-emepụta tube nke na-atụgharị n'ime ime ka ọ bụrụ ihe atụ na-ekpo ọkụ na mmiri mmiri nitrogen, na silica gel pad na nozzle enhances the sealing of the frizing tube.
3. Ahịrị ọkpọ ọkpọ dị mfe iji gbanwee mkpuchi.
4. A na-emepụta ọkpọkọ ọkpọkọ na ọkpọkọ ọkpọkọ na ihe ọkụkụ PP nke otu batch na ihe nlereanya, ya mere otu mgbasawanye mgbasa ozi na-eme ka a hụ na enwere ike mechie ha na okpomọkụ ọ bụla.
5. Nnukwu akara akara dị mma maka ide ihe.
6. The tube bụ nnọọ transperent na mfe ịhụ sample.
7. Nhazi ala gburugburu dị mma maka ịwụsa mmiri mmiri na ibelata ihe fọdụrụ.
Ndị a bụ isi ihe ọmụma gbasara okpu mkpọda n'ime nke tube nke cryopreservation.Enwere m olileanya na ọdịnaya dị n'elu nwere ike inyere gị aka.Daalụ maka ikiri na nkwado gị.A ga-edozira gị ozi ọzọ ma emechaa.Biko ṅaa ntị na mmelite webụsaịtị anyị.
Ndị a bụ isi ihe ọmụma gbasara okpu mkpọda n'ime nke tube nke cryopreservation.Enwere m olileanya na ọdịnaya dị n'elu nwere ike inyere gị aka.Daalụ maka ikiri na nkwado gị.A ga-edozira gị ozi ọzọ ma emechaa.Biko ṅaa ntị na mmelite webụsaịtị anyị.
Oge nzipu: Sep-15-2022