Usoro ojiji ziri ezi na usoro nke pipette serological

A na-ejikarị pipette serological, nke a makwaara dị ka pipette disposable, iji tụọ oke mmiri mmiri nke ọma, nke a ga-eji ya na pipette kwesịrị ekwesị.Pipette bụ ngwaọrụ nlegharị anya nke a na-eji nyefe otu olu ngwọta nke ọma.Pipette bụ ihe eji atụ ihe, nke a na-eji naanị tụọ olu nke ngwọta ọ na-ewepụta.Ọ bụ tube iko dị ogologo ma dị mkpa nke nwere nnukwu mgbasawanye n'etiti.Ngwunye ala ya dị n'ụdị ọnụ dị nkọ, a na-eji eriri akara kanye olu olu nke elu, nke bụ ihe ịrịba ama nke kpọmkwem ụda a ga-emegharị.

Usoro ojiji nke ọma na usoro nke serum pipette:

1. Tupu ojiji: Mgbe ị na-eji pipette, buru ụzọ lelee akara pipette, ọkwa ziri ezi, ọnọdụ akara akara, wdg.

2. Aspiration: Jide elu elu nke pipette na mkpịsị aka na mkpịsị aka etiti nke aka nri gị, ma tinye ọnụ ala nke pipette n'ime ngwọta a ga-aṅụ.Ntinye ekwesịghị ịbụ nke na-emighị emi ma ọ bụ dị omimi, na-abụkarị 10 ~ 20mm.Ọ bụrụ na ọ na-emighị emi nke ukwuu, ọ ga-ebute mmiri ara.Ọchịchọ nke ngwọta n'ime bọọlụ ịsacha ntị ga-emerụ ihe ngwọta ahụ.Ọ bụrụ na ọ dị omimi, ọ ga-arapara nnukwu ngwọta n'èzí tube.Were aka ekpe were bọl na-asa ntị, jikọọ ya na ọnụ elu nke tube ma jiri nwayọọ nwayọọ kpoo ngwọta ahụ.Buru ụzọ kukuo ihe dị ka 1/3 nke olu tube ahụ.Jiri mkpịsị aka aka nri pịa ọnụ tube ahụ, wepụ ya, jide ya n'ahịrị, ma gbanwee tube ahụ iji mee ka ngwọta ahụ kpọtụrụ akụkụ dị n'elu ọnụ ọgụgụ iji dochie mmiri na mgbidi dị n'ime.Mgbe ahụ, wepụ ihe ngwọta ahụ n'ọnụ ala nke tube ma tụfuo ya.Mgbe ịsachara ugboro atọ, ị nwere ike ịmịnye ngwọta ahụ ihe dịka 5mm n'elu ọnụ ọgụgụ.Ngwa ngwa pịa ọnụ tube na mkpịsị aka nri nke aka nri.

3. Gbanwee ọkwa mmiri mmiri: bulie pipette elu na pụọ ​​na ọkwa mmiri mmiri, kpochapụ mmiri mmiri na mgbidi dị n'èzí nke pipette na akwụkwọ nzacha, njedebe nke tube na-adabere na mgbidi dị n'ime nke ihe ngwọta, tube. ahu na-anọgide vetikal, ubé izuike mkpịsị aka ndeksi na-eme ka ihe ngwọta na tube ji nwayọọ nwayọọ na-asọpụta si n'ọnụ ala, ruo mgbe ala nke meniscus nke ngwọta bụ tangent na akara, na ozugbo pịa tube ọnụ na index mkpịsị aka.Wepu mmiri mmiri na-ekpuchi mgbidi ahụ, wepụ ya na pipette, ma tinye ya n'ime ụgbọ mmiri na-enweta ihe ngwọta.

4. Ntupu nke ngwọta: Ọ bụrụ na arịa na-enweta ihe ngwọta bụ a conical flask, na conical flask kwesịrị tilted 30 Celsius.Pipette nke a na-atụfu ya kwesịrị ịbụ vetikal.Ngwunye ala nke tube kwesịrị ịdị nso na mgbidi dị n'ime nke flask conical.Tọpụ mkpịsị aka mkpịsị aka ahụ ma mee ka ngwọta ahụ jiri nwayọọ nwayọọ na-agbada mgbidi karama ahụ.Mgbe ọkwa mmiri na-adaba na isi iyi ahụ, tube na-akpọtụrụ mgbidi dị n'ime nke karama ahụ maka ihe dịka 15 sekọnd, wee wepụ pipette.Obere ihe ngwọta fọdụrụ na njedebe nke tube ekwesịghị ịmanye ka ọ pụta, n'ihi na a na-eburu n'uche olu nke ngwọta na-ejide na njedebe.