Enwere ike ijikwa tubes ultrafiltration centrifuge?Nke a bụ azịza

Igwe centrifuge tube bụ tube dị mfe nke nwere ike iguzogide ọsọ na nrụgide dị elu, dị ka ikewapụ ụfọdụ ihe atụ na ikewapụ sediments dị elu.Igwe ikuku ultrafiltration centrifuge nwere akụkụ abụọ yiri tube dị n'ime na tube dị n'èzí.Ọkpụkpọ dị n'ime bụ akpụkpọ anụ nwere ụfọdụ ibu molekụla.N'oge centrifugation dị elu, ndị nwere obere molecular arọ ga-adaba n'ime obere tube (ya bụ n'èzí tube), na ndị nwere ibu molekụla buru ibu ga-ejide n'ime tube elu (ya bụ n'ime tube).Nke a bụ ụkpụrụ nke ultrafiltration na a na-ejikarị etinye uche n'omume.

Enwere ike iji tubes centrifuge Ultrafiltration na-enweghị pretreatment, mana maka nhazi ihe nlele protein, ọkachasị maka ngwọta protein dilute (<10ug / ml), ọnụọgụ mgbake nke itinye uche na membranes ultrafiltration anaghị abụkarị ọnụọgụ.Ọ bụ ezie na ihe PES na-ebelata mgbasa ozi na-enweghị isi, ụfọdụ protein, karịsịa mgbe ha na-agbaze, nwere ike inwe nsogbu.Ogo nke njikọ na-enweghị nkọwa dịgasị iche na nhazi nke protein n'otu n'otu.Protein nwere ngalaba ebubo ebubo ma ọ bụ hydrophobic yikarịrị ka ọ ga-ejikọta ya na elu dị iche iche.Pretreatment passivation n'elu nke ultrafiltration centrifuge tube nwere ike ibelata mfu nke protein adsorption n'elu akpụkpọ ahụ.N'ọtụtụ ọnọdụ, pretreatment nke kọlụm tupu itinye uche na ngwọta protein dilute nwere ike imeziwanye ọnụego mgbake, n'ihi na ngwọta ahụ nwere ike mejupụta saịtị mgbasa ozi protein na-ekpuchi anya na akpụkpọ ahụ na elu.Usoro ngafe bụ ịkwanye kọlụm ahụ na oke olu nke ngwọta passivation maka ihe karịrị awa 1, sachaa kọlụm ahụ nke ọma na mmiri distilled, wee kpoo ya otu ugboro na mmiri distilled iji wepụ ihe ngwọta passivation nke nwere ike ịnọgide na fim ahụ kpamkpam. .Kpachara anya ka ị ghara ikwe ka ihe nkiri ahụ kpọọ nkụ mgbe ngafe gasịrị.Ọ bụrụ na ịchọrọ iji ya emechaa, ịkwesịrị ịgbakwunye mmiri distilled na-adịghị mma iji mee ka ihe nkiri ahụ dị mmiri.

Enweghị ike ịgba ọgwụ mgbochi mmiri ma jiri ya mee ihe ọzọ.Ebe ọ bụ na ọnụahịa nke otu tube adịghị ọnụ ala, ọtụtụ ndị na-agbalị iji ya mee ihe - ahụmahụ ahụ bụ iji kpochapụ akpụkpọ anụ ahụ na mmiri distilled ruo ọtụtụ oge na centrifuge ya otu ugboro ma ọ bụ ugboro abụọ.Obere tube nke nwere ike ịmegharị na ntụgharị nwere ike ịbanye n'ime mmiri mmiri na-ekpo ọkụ wee gbanye ya ọzọ maka oge ọzọ, nke ga-aka mma.Enwere ike iji ya mee otu ihe ahụ ugboro ugboro, a pụkwara itinye ya na mmiri mmiri na-ekpo ọkụ mgbe ọ naghị eji ya, ma a ga-egbochi nje bacteria.Ejikọtala ihe atụ dị iche iche.Ụfọdụ ndị mmadụ na-ekwu na ịṅụ mmanya 20% na 1n NaOH (sodium hydroxide) nwere ike igbochi uto nke nje bacteria na igbochi nkụ.Ọ bụrụhaala na akpụkpọ ahụ ultrafiltration na-awakpo mmiri, a pụghị ịhapụ ya ka ọ kpọọ nkụ.Otú ọ dị, ndị ọzọ na-ekwu na ọ ga-ebibi usoro akpụkpọ ahụ.N'ọnọdụ ọ bụla, ndị na-emepụta ihe anaghị akwado ijikwa ya ọzọ.Ojiji ugboro ugboro ga-egbochi nha pore nke akpụkpọ ahụ nzacha, na ọbụna mee ka mmiri na-agbapụta mmiri, nke ga-emetụta nsonaazụ nnwale.